Chemistry: electrical and wave energy – Apparatus – Electrolytic
Patent
1984-05-07
1988-07-19
Tung, T.
Chemistry: electrical and wave energy
Apparatus
Electrolytic
204 1T, 435 26, 435288, 435817, G01N 2746
Patent
active
047583231
ABSTRACT:
This specification discloses methods of detection or measurement of an enzyme or of its specific substrate, and sensors used in such methods. The present invention is concerned with a multi-enzyme system, and the specification discloses, as one aspect of the invention a method of assay in which an electrode poised at a suitable potential is contacted with a system comprising a first enzyme, a cofactor linked with said enzyme and a mediator compound which transfers charge to the electrode from the first enzyme when its electrical state is changed by reaction of cofactor material.
The cofactor may be NAD, NADP (both collectively referred to herein as NAD(P)), cAMP, ATP, GTP, TTP, or CTP.
The specification particularly illustrates a method of assay in which an electrode (1) poised at a suitable potential is contacted with a system comprising a first enzyme E.sub.1 a nicotinamide adenine dinucleotide compound N linked with said enzyme E.sub.1 and a mediator compound F which transfers charge to the electrodes from the first enzyme when its electrical state is changed by a NAD(P) NAD(P)H reaction. The NAD compound may act as a "bridge" between the said enzyme/mediator system and further NAD utilizing enzyme E2.
REFERENCES:
patent: 4224125 (1980-09-01), Nakamura et al.
patent: 4225410 (1980-09-01), Pace
patent: 4271265 (1981-06-01), Deneke et al.
patent: 4318980 (1983-11-01), Boguslaski et al.
patent: 4376689 (1983-03-01), Nakamura et al.
patent: 4416983 (1983-11-01), Roder et al.
patent: 4427771 (1984-01-01), Misaki et al.
patent: 4446231 (1984-05-01), Self
patent: 4463090 (1984-07-01), Harris
patent: 4490464 (1984-12-01), Gorton et al.
patent: 4545382 (1985-10-01), Higgins et al.
Davis Graham
Hill Hugh A. O.
Genetics International, Inc.
Tung T.
LandOfFree
Assay systems using more than one enzyme does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assay systems using more than one enzyme, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay systems using more than one enzyme will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-595647