Assay systems based on magnesium-responsive enzymes

Details Assay systems based on magnesium-responsive enzymes Assay systems based on magnesium-responsive enzymes

1983-05-05

1987-04-14

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 15, 435 17, 435 21, 435 24, 435 26, C12Q 154, C12Q 100, C12Q 148, C12Q 150, C12Q 142, C12Q 136, C12Q 132

Patent

active

046578544

ABSTRACT:
There is provided an assay method for determining the concentration of magnesium ions in biological fluids. The method comprises the introduction of a sample of biological fluid to a magnesium-free assay solution containing at least one reactant and an enzyme capable of catalyzing a reaction with at least one reactant wherein the enzyme activity, and hence the rate of reaction, is partially or totally dependent on and correlatable to the concentration of magnesium that is present. The rate of reaction is measured by monitoring the loss of a detectable reactant or the formation of a detectable product.

REFERENCES:
patent: 3929580 (1975-12-01), Forgione et al.

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