Assay systems and methods for measuring P38 map kinase, and modu

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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4353201, 4352523, 435325, C12Q 168, C12N 1563, C12N 121, C12N 510

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active

060108568

ABSTRACT:
Assay systems utilizing a reporter construct based on p38 phosphorylation of myocyte enhancer factor 2 (MEF2) transcription factor are provided. The reporter construct and methods of use are provided, as well as methods of treatment of inflammatory and muscular disorders associated with MEF2-regulated gene expression.

REFERENCES:
J. Han et al., "Activation of the Transcription Factor MEF2C by the MAP Kinase p38 in Inflammation", Nature 386:296-299, Mar. 1997.
Y. Jiang et al., "Characterization of the Structure and Function of a New Mitogen-Activated Protein Kinase (p38.beta.)", J. Biol. Chem. 271: 17920-6, Jul. 1996.

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