Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2011-04-19
2011-04-19
Pak, Michael (Department: 1646)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
Reexamination Certificate
active
07927820
ABSTRACT:
The invention provides an assay system for detecting a molecule that interacts with a membrane channel, the assay system including cell membranes including one or more membrane channels; support bodies including scintillant and a coupling agent that associates with the cell membrane; a ligand that is selected to bind the membrane channel, the ligand including a scintillant-activating label. According to the invention, association of the support bodies with the cell membrane and binding of the ligand to the membrane channel results in emission from the scintillant of the support bodies, and, in the presence of a test molecule that interacts with the membrane channel, the emission from the scintillant of the support bodies changes. Methods of identifying a molecule that interacts with a cell membrane channel are also described.
REFERENCES:
patent: 4568649 (1986-02-01), Bertoglio-Matte
patent: 2003/0166099 (2003-09-01), Sabbadini et al.
patent: 2007/0031814 (2007-02-01), Roos et al.
patent: 154734 (1985-09-01), None
patent: WO 02/05860 (2002-01-01), None
patent: WO 03/021271 (2003-03-01), None
International Search Report dated Jan. 5, 2006 for corresponding Appln. No. PCT/US2005/025428.
Cooper, Matthew A.: “Advances in Membrane Receptor Screening and Analysis” Journal of Molecular Recognition, vol. 17, No. 4, Jul. 2004, pp. 286-315, XP002359417.
Langmead, Christopher J. et al.: “Characterisation of the Binding of (3H)-SB-674042, a novel nonpeptide antagonist, to the human orexin-1 receptor” British Journal of Pharmacology, vol. 141, No. 2, Jan. 2004, pp. 286-315 XP002359417.
International Preliminary Report on Patentability for related application PCT/US2005/025428, issued Jan. 23, 2007.
International Search Report and Written Opinon for related application PCT/US2005/025428, mailed Jan. 5, 2006.
Cornell et al., “A biosensor that uses ion-channel switches,”Nature, 1997: 387, pp. 580-583.
Denyer et al., “HTS approaches to voltage-gated ion channel drug discovery,”Drug Discovery Today, 1998: 3, pp. 323-332.
Hillyard et al., “A New Conus Peptide Ligand for Mammalian Presynaptic Ca2+Channels,”Neuron, 1992: 9, pp. 69-77.
Mintz et al., “P-Type Calcium Channels in Rat Central and Peripheral Neurons,”Neuron, 1992: 9, pp. 85-95.
Neher, “Ion Channels for Communication between and within Cells,”Neuron, 1992: 8, pp. 605-612.
Olivera et al., “Neuronal Calcium Channel Antagonists. Discrimination between Calcium Channel Subtypes using ω-Conotoxin fromConus magusVenom,”Biochemistry, 1987: 26, pp. 2086-2090.
Randall et al., “Pharmacological Dissection of Multiple Types of Ca2+Channel Currents in Rat Cerebellar Granule Neurons,”J. Neurosci., 1995: 15, pp. 2995-3012.
Sasaki et al., “Combinatorial synthesis of ω-Conotoxin MVIIC analogues and their binding with N- and P/Q-type calcium channels,”FEBS Letters, 2000: 466, pp. 125-129.
Wheeler et al., “Roles of N-Type and Q-Type Ca2+Channels in Supporting Hippocampal Synaptic Transmission,”Science, 1994: 264, 107-111.
Whorlow et al., “Selectivity of ω-Conotoxin GVIA for N-Type Calcium Channels in Rat Isolated Small Mesenteric Arteries,”Clin. Exp. Pharmacol Physiol., 1996: 23(1), pp. 16-21.
Xu et al., “Ion-channel assay technologies: quo vadis?,”Drug Discovery Today, 2001: 6, pp. 1278-1287.
Zhang et al., “Functional studies of bradykinin receptors in Chinese hampster ovary cells stably expressing the human B2bradykinin receptor,”Int. lmmunopharmacol, 2001: 1, pp. 955-965.
Zhang et al., “A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays,”J. Biomol. Screening, 1999: 4, pp. 67-73.
Codd Ellen E.
Zhang Sui-Po
Janssen Pharmaceutica NV
Pak Michael
LandOfFree
Assay systems and methods for detecting molecules that... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assay systems and methods for detecting molecules that..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay systems and methods for detecting molecules that... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2716009