Assay system

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Details

C435S091100, C435S091200

Reexamination Certificate

active

06544747

ABSTRACT:

TECHNICAL FIELD
The present invention relates, in general, to an assay system and, in particular, to a molecular assay system for the detection and/or quantification of mouse T cell receptor excision circles. The present system can be used to detect newly produced naïve T lymphocytes.
BACKGROUND
T cell receptor excision circles (TRECS) are episomal DNA circles that are generated by the DNA recombination process that is used by T lymphocytes to produce antigen-specific T cell receptors. The non-replicated DNA circles are diluted out with cell division. The present invention provides a method of assaying for newly generated mouse naïve T lymphocytes based on the detection of signal joint TRECs.
SUMMARY OF THE INVENTION
The present invention relates generally to a molecular assay system for the detection and/or quantification of mouse T cell receptor excision circles. More particularly, the invention relates to a method of assaying for newly produced mouse naïve T lymphocytes.
Objects and advantages of the present invention will be clear from the description that follows.


REFERENCES:
Al-Harthi et al., “Detection of T cell receptor circles (TRECs) as biomarkers for de novo T cell synthesis using a quantitative polymerase chain reaction-enzyme linked immunosorbent assay (PCT-ELISA)”, Journal of Immunological Methods 237: 187-197 (2000).
Sodora et al, “Quantification of thymic function by measuring T cell receptor excision circles within peripheral blood and lymphoid tissues in monkeys”, Eur. J. Immunol. 30:1145-1153 (2000).
Douek et al., “Changes in thymic function with age and during the treatment of HIV infection”, Nature 396:690-695 (1988).
Sempowski et al., “T Cell Receptor Excision Circle Assessment of Thymopoiesis in Aging Mice”, Mol. Immunol. 38:841 (2001).

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