Assay processes and materials therefor

Details Assay processes and materials therefor Assay processes and materials therefor

1983-09-27

1987-01-20

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436526, 436530, 436536, 436537, 436800, 436808, 436815, 436817, 436825, 436826, 436546, 422 61, G01N 33542, G01N 33544, G01N 33553

Patent

active

046379851

ABSTRACT:
A process of carrying out a specific binding assay, for the qualitative detection or quantitative or semi-quantitative determination of an analyte which forms a component of a specific binding reaction, using a labelled conjugate form of a derivative or analogue of one of the relevant specific binding partners, said labelled form being capable of emitting delayed luminescence upon photo-excitation, characterized in that the labelled conjugate form of one of the relevant specific binding partners comprises a derivative or analogue of the specific binding partner with a derivative of a luminescent substance which shows a delayed light emission upon photoexcitation, the delayed light emission of which is subject to quenching by molecular oxygen, and characterized further in that the assay is carried out by the use of a time-resolved photometric method in the presence of an effective amount of a quench-inhibiting substance able to prevent quenching by molecular oxygen.

REFERENCES:
patent: 4160818 (1979-07-01), Smith et al.
patent: 4252783 (1981-02-01), Kam et al.
patent: 4492762 (1985-01-01), Wang et al.
Immuno Assays for the 80s, Voller et al, Eds., University Park Press, Baltimore, 1981, p. 96.

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