Assay process with non-boiling denaturation

Details Assay process with non-boiling denaturation Assay process with non-boiling denaturation

1981-03-30

1983-11-29

Nucker, Christine M.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436804, 436825, G01N 3336, G01N 3360

Patent

active

044181515

ABSTRACT:
Competitive protein binding radioassay for sera (or cell) vitamin B.sub.12 and/or serum folate (target components, or analytes) or other target components in liquid ambient utilizing a highly alkaline (pH 12-14) environment and reducing agent for denaturing separating the target component(s) from serum without boiling, consistent with other requirements of such assays, then reducing pH to an 8-10 range for effecting the competitive protein binding after which protein bound and unbound groups of radioactively tagged replicates of the target component(s) can be separated and detected to determine content of the target component(s) in the original serum (or cell), i.e. original endogenous analyte(s).

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