Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1984-09-27
1989-02-14
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 435810, 436518, 436536, 436538, 436805, G01N 33535, G01N 33543, G01N 33536
Patent
active
048046256
ABSTRACT:
Binding assay methods involving determining the presence of analytes in samples through enzymatic formation of detectable substances in amounts related to the amount of analyte present in the sample and monitoring for the presence of the substances in distinct phases. Methods according to the invention include use of labelled materials which associate with the analyte to be determined or compete with the analyte for association with an added binder. The labelled materials employed include label portions which enzymatically form substances from substrates provided in or existing as a first phase, or, upon enzymatic treatment in a first phase, disassociate into substances capable of existing in or as a second distinct phase. Formation of the detectable substances is monitored by determining the transfer of the substance to a second distinct phase in contact with the first phase or by determining formation of a second distinct phase. The assays are useful in determining human IgG protein in blood samples and other constituents of blood or other biological samples without elaborate instrumentation, allowing for practice outside the clinical laboratory.
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Heller Michael J.
Morrison Larry E.
Royer Garfield P.
Amoco Corporation
Janiuk Anthony J.
Kepplinger Esther M.
Magidson William M.
Medhurst Ralph C.
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