Assay preparation containing capture and detection polynucleotid

Details Assay preparation containing capture and detection polynucleotid Assay preparation containing capture and detection polynucleotid

1995-01-31

1997-09-02

Naff, David M.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435174, 435181, 436532, 530816, C07H 2104, C12N 1106, G01N 33549, C12Q 168

Patent

active

056633181

ABSTRACT:
Heterofunctional crosslinking agents are used to produce a device for detecting a target polynucleotide. Synthetic single stranded capture and detector polynucleotides are covalently immobilized to plastic surfaces by novel heterofunctional reagents. The capture and detector polynucleotides are covalently attached through a synthetic region of amine-bearing nucleotides that interact with reactive moieties on the heterofunctional crosslinker. The regions of the capture and detector polynucleotides that can hybridize a diagnostically important target polynucleotide are protected from binding to a solid phase support by intrastrand pairing. This system provides monolayers of immobilized polynucleotides with precise orientation and unimpaired diagnostic coding regions.

REFERENCES:
patent: 4657873 (1987-04-01), Gadow et al.
patent: 4808530 (1989-02-01), Means et al.
patent: 5002883 (1991-03-01), Bieniarz et al.
patent: 5200313 (1993-04-01), Carrico
patent: 5200314 (1993-04-01), Urdea
patent: 5273882 (1993-12-01), Snitman et al.
patent: 5279955 (1994-01-01), Pegg
patent: 5312728 (1994-05-01), Lizardi et al.
patent: 5328825 (1994-07-01), Warren, III et al.
patent: 5436147 (1995-07-01), Pegg et al.

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