Assay of vitamin B12

Details Assay of vitamin B12 Assay of vitamin B12

1981-09-09

1984-01-17

Nucker, Christine M.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436825, G01N 3354, G01N 3356, G01N 3358

Patent

active

044264550

ABSTRACT:
A method of assaying for vitamin B12 achieves denaturation of serum binding proteins which normally bind the vitamin B12 by treatment with alkali at ambient temperature. The assay is characterized by the use, in the alkali denaturation step, of a dithiopolyol and cyanide, and by the use, during the intrinsic factor assay step, of a vitamin B12 analogue such as cobinamide to bind with any remaining serum proteins. The invention also includes a kit, in which the dithiopolyol is provided in admixture with the alkali.

REFERENCES:
patent: 4028465 (1977-06-01), Lewin et al.
patent: 4279859 (1981-07-01), Gutcho et al.
patent: 4332786 (1982-06-01), Cabelli et al.

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