Assay of salicylates or reduced pyridine nucleotides

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 13, 435 14, 435 19, 435 22, 435 23, 435 26, 435805, 435810, C12Q 126, C12Q 100, C12Q 156, C12Q 154

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054609488

ABSTRACT:
A method for the estimation of a salicylate in a sample is characterized by enzymatically converting the salicylate to a catechol by the action of a salicylate mono-oxygenase enzyme on the salicylate in the presence of a reduced pyridine nucleotide, reacting the catechol with a compound selected from compounds of formula I or amine or phenolic compounds of formulae II, III or IV ##STR1##

REFERENCES:
patent: 4416983 (1983-11-01), Roder et al.
Analyst, Sastry et al, vol. 110, Apr. 1985, p. 395.
Longenecker et al Clin. Chem., vol. 30, No. 8, 1984, pp. 1369-1371.
Clinica Chimica Acta, Chubb et al, vol. 155, No. 3, Mar. 28, 1986, pp. 209-220.
Clinica Chimica Acta, vol. 155, No. 3, 1986, S. A. P. Chubb, et al, "An Enzyme Mediated, Colorimetric Method For The Measurement Of Salicylate" pp. 209-220.
Analyst, vol. 110, No. 4, 1985, C. S. Sastry et al, "Spectrophotometric Methods For The Determination Of O-Dihydroxybenzene Derivatives", pp. 395-398.
Clin. Chem., vol. 30, Nov. 8, 1984, Longenecker et al, "A Tableted Enzymic Reagent For Salicylate, For Use In A Discrete Multiwavelength Analytical System (Paramax.TM.)", pp. 1369-1371.

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