Assay of peroxidatively active materials

Details Assay of peroxidatively active materials Assay of peroxidatively active materials

1979-03-08

1981-05-26

Gron, Teddy S.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

23230B, 252408, 252186, 2521883CL, 25230116, 422 55, 422 56, 422 57, 422 61, 424 7, 424 8, 424 12, 435 14, 435 28, 435188, G01N 3114, C09K 300, G01N 3122

Patent

active

042699380

ABSTRACT:
A new peroxidase assay for substances demonstrating peroxidative activity is described which is based on the peroxidase catalyzed oxidation of dichlorofluorescin to dichlorofluorescein by peroxide. The dichlorofluorescin is formed from diacetyldichlorofluorescin either by the direct addition of peroxide or by the inclusion of a peroxide generating system (e.g., glucose plus glucose oxidase or uric acid and uricase) in the reaction mixture. As long as all reactants other than the peroxidase are present in excess, the rate of fluorescence increase is linearly related to the amount of peroxidase in the sample under assay.

REFERENCES:
patent: 3654090 (1972-04-01), Schuurs et al.
patent: 3791932 (1974-02-01), Schuurs et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3964870 (1976-06-01), Tiedemann
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4042335 (1977-08-01), Clement
patent: 4069016 (1978-01-01), Wu
patent: 4089747 (1978-05-01), Bruschi
Keston, A. et al., Anal. Biochem., vol. 11, pp. 1-5 (1965).
Brandt, R. et al., Anal. Biochem., vol. 11, pp. 6-9 (1965).
Black, M. J. et al., Anal. Biochem., vol. 58, pp. 246-254 (1974).

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