Assay of micro-organisms in cell cultures

Details Assay of micro-organisms in cell cultures Assay of micro-organisms in cell cultures

2005-10-04

2005-10-04

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S288700

Reexamination Certificate

active

06951723

ABSTRACT:
Micro-organisms which over-produce ATPase, especially mycoplasmas in a sample of a cell culture, are detected by making use of the ATPase activity to convert cellular or externally added ATP to ADP. The decrease in ATP externally added or the increase or absolute level of ADP produced from cellular ATP can then be detected or measured. For example, if a reagent such as phosphenol pyruvate/pyruvate kinase is added to convert that ADP back to ATP, the resulting high level of ATP can be detected bioluminescently using the reaction: Luciferin+luciferase enzyme+ATP→hv+products.

REFERENCES:
patent: 2323167 (1998-09-01), None
Written Opinion mailed Aug. 24, 2001 in PCT/GB/00/04264.

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