Assay of iron and iron binding protein reagents and methods

Details Assay of iron and iron binding protein reagents and methods Assay of iron and iron binding protein reagents and methods

1978-09-07

1980-09-23

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

23230R, 252408, G01N 3316

Patent

active

042240344

ABSTRACT:
The discovery of 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine and its sulfonated form, having particular advantages when used as both a chelator and indicator of ferrous ions in organic or aqueous mediums; a process for the synthesis of those compounds in which hydrazine is used as both a reactant and as a solvent in production of intermediate compounds, eliminating the need of ethanol and the disadvantageous ether-extraction step; the use of N,N-dimethylformamide as a reaction solvent in the production of triazines; the use of the new triazine compounds to measure iron, transferrin, and UIBC/TIBC in biological systems; the use of dimethylsulfoxide and certain processes to eliminate the interferences commonly encountered in these assays; and the use of the triazine compounds to measure both total and/or reduced iron in a variety of matrices.

REFERENCES:
patent: 3887332 (1975-06-01), Takase
patent: 3925020 (1975-12-01), Ogawa
patent: 4072627 (1978-02-01), Gindler
patent: 4111657 (1978-09-01), Denney

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