Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is a biological cell or cell fragment
Patent
1983-05-20
1985-12-17
Marantz, Sidney
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is a biological cell or cell fragment
435 7, 435 29, 436513, 436808, G01N 3354, C12Q 129
Patent
active
045593108
ABSTRACT:
The present invention is directed to an in vitro assay, useful in determining the effectiveness of anti-allergy compounds and/or useful in measuring the degree of sensitivity of a patient to particular allergens.
The present invention permits potential anti-allergy agents to be assayed in a number of ways. For example, the binding and dissociation rates of IgE to the mast cells in the presence and the absence of the substance being tested may be measured thereby giving a direct indication of that substance's ability to interfere with the IgE binding reaction. Another measure of a substance's potential as an anti-allergy agent is based upon the release of mediators or other compounds from the mast cells after sensitization by the allergen and exposure of the sensitized cells to the allergen.
The present invention generally involves the following steps:
REFERENCES:
Daeron, et al., Journal of Immunology, 129 (3), pp. 1212-1218 (1982).
"Basic and Clinical Immunology," D. P. Stites et al., eds., Lange Medical Publications, Los Altos, Ca., 1984 pp. 246-253.
Guyton, Textbook of Medical Physiology, 77 et seq. (5th Ed. 1976).
L. J. Roberts et al., Biochem. Biophys. Acta 575:185 (1979).
Nagas et al., Science 212:333 (1981).
F. Lui et al., J. Immunol. 124:2728 (1980).
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Shaff et al., Anal. Biochem. 94:425 (1979).
Robinson et al., Biochem. J. 107:321 (1968).
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Cantor Harvey I.
Nabel Gary
Conlin David G.
Dana Farber Cancer Institute
Krawczewicz Louanne C.
Linek Ernest V.
Marantz Sidney
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