Assay methods and systems

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S023100, C536S024320, C435S006120, C435S091200

Reexamination Certificate

active

07122659

ABSTRACT:
Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second reagent reacts with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent. A polyion is introduced into at least one of the first and second reagent mixtures, and the fluorescent polarization in the second reagent mixture relative to the first reagent mixture is determined, this fluorescent polarization being indicative of the rate or extent of the reaction.

REFERENCES:
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5527688 (1996-06-01), Mallia
patent: 5587285 (1996-12-01), Cloyd et al.
patent: 5646001 (1997-07-01), Terstappen et al.
patent: 5665865 (1997-09-01), Lerner et al.
patent: 5688648 (1997-11-01), Mathies et al.
patent: 5707804 (1998-01-01), Mathies et al.
patent: 5728528 (1998-03-01), Mathies et al.
patent: 5744305 (1998-04-01), Fodor et al.
patent: 5747247 (1998-05-01), Kowalczykowski et al.
patent: 5759787 (1998-06-01), Strulovici
patent: 5779868 (1998-07-01), Parce et al.
patent: 5783687 (1998-07-01), Glazer et al.
patent: 5804395 (1998-09-01), Schade et al.
patent: 5853992 (1998-12-01), Glazer et al.
patent: 5858195 (1999-01-01), Ramsey
patent: 5869255 (1999-02-01), Mathies et al.
patent: 5885470 (1999-03-01), Parce et al.
patent: 5925558 (1999-07-01), Tsien et al.
patent: 6071748 (2000-06-01), Modlin et al.
patent: 6087107 (2000-07-01), Sheffield et al.
patent: 6100039 (2000-08-01), Burke et al.
patent: 6149787 (2000-11-01), Chow et al.
patent: 6153073 (2000-11-01), Dubrow et al.
patent: 6203994 (2001-03-01), Epps et al.
patent: 6287774 (2001-09-01), Nikiforov
patent: 6326142 (2001-12-01), Royer
patent: 6436646 (2002-08-01), Nikiforov
patent: 6472141 (2002-10-01), Nikiforov
patent: 6503709 (2003-01-01), Bekkaoui et al.
patent: 2002/0061546 (2002-05-01), Tsien et al.
patent: 0 781 853 (1997-07-01), None
patent: 786 666 (1997-07-01), None
patent: WO-9412665 (1994-06-01), None
patent: WO-95/15981 (1995-06-01), None
patent: WO-98/00231 (1998-01-01), None
patent: WO-98/00705 (1998-01-01), None
patent: WO-98/18956 (1998-05-01), None
patent: WO-98/45481 (1998-10-01), None
patent: WO-99/16162 (1999-04-01), None
patent: WO-99/23466 (1999-05-01), None
patent: WO-9964840 (1999-07-01), None
patent: WO-00/23785 (2000-04-01), None
Hawley's Condensed Chemical Dictionary, p. 236, 237, 12th Edition, Van Nostrand Reinhold, New York (1993).
Whatman Product Guide, Pure Cellulose Grades) pp. 65, 66 (1995).
Andersson, L. et al., “Isolation of Phosphoproteins by Immobilized Metal(Fe3+) Affinity Chromoatography,” Anal Biochem. (1986) 154:250-254.
Checovich, W.J. et al., “Fluorescence Polarization-a New Tool for Cell and Molecular Biology,” Nature (1995) 75:254-256.
Chen, W. et al., “Fluorescence Polarization in Homogeneous Nucleic Acid Analysis,” Genome Res. (1999) 9:492-8, May.
Dandliker, B.B. et al., “Quantification of the Antigen-Antibody Reaction by the Polarization of Fluorescence,” Biochem Biophys Res Commun. (1961) 5:299-304.
Dandliker, B.B. et al., “Fluorescence Polarization in Immunochemistry,” Immunochemistry (1970) 7:799.
Dandliker, B.B. et al., “Fluorescence Polarization Immunoassay. Theory and Experimental Method,” Immunochemistry (1973) 10:219.
Deshpande, S.S. et al., “A Robust, Versatile Tyrosine Kinase Assay for HTS in Drug Discovery,” Prog. Biomed. Optics (SPIE) (1999) 3603:251-61.
Devlin, R. et al., “Homogenous Detection of Nucleic Acids by Transient-State Polarized Fluorescence,” Clin. Chem. (1993) 39:1939.
Egholm, M. et al.,Nature(1993) 365:566-568.
Evett, J. et al., “DNA-Polylysine Interaction as Studied by Polarization of Fluorescence,” Ann. N.Y. Acad. Sci. (1969) 158:210-22.
Gibson, et al., “A Homogeneous Method for Genotyping with Fluorescence Polarization,” Clinical Chemistry (1997) 43:1336-41.
Gingeras, T.R. et al., “A Spectrofluorometric Method for Measurement of Restriction Endonuclease Activity Based on Fluorescence Polarization,” J. Cell. Biochem. Suppl. (1992) Suppl. 17 Part C, p. 173.
Glotov, B.O. et al., “Histone H1-DNA Interaction of Phosphorylation on the Interaction of Histone H1 with Linear Fragmented DNA,” Nucleic Acids Research (1977) 4:1065:82.
Green, R.C. “Fluorescence Polarization Colloid Charge Titration: Development and Application for Feed Forward Coagulant Control at Water Treatment Facilities,” Ph.D. Thesis, University of Colorado (1997).
Ichimura, S. et al., “The Interaction of 8-Anilino-1-Naphthalanesulfonatate with Polylysine and Plyarginine,” Biopolymers (1977) 16:1449-64.
Jiskoot, W. et al., “Preparation and Application of a Fluorescein-labled Peptide for Determining the Affinity Constant of a Monoclonal Antibody-Hapten Complex by Fluorescence Polarization,” Anal. Biochem. (1991) 196:421.
Jolley, M.E. et al., “Fluorescence Polarization Immunoassay I. Monitoring Aminoglycoside Antibiotics in Serum and Plasma,” Clinical Chemistry (1981) 27:1190-97.
Jolley, M.E. et al., “Fluorescence Polarization Assays for the Detection of Proteases and Their Inhibitors,” Journal of Biomolecular Screening (1996) 1:33-38.
Jorden, R.M. et al., “Colloid Charge Capacity-Its Measurement, Problems and Promise,” Chemical Water and Wastewater Treatment IV (1997) 121-35.
Kumke, M.U. et al., “Hybridization of Fluorescein-Labeled DNA Oligomers Detected by Fluorescence Anisotropy with Protein Binding Enhancement,” Anal. Chem. (1995) 67:3945-51.
Laurence, D.J.R., “A Study of the Adsorption of Dyes on Bovine Serum Albumin by the Method of Polarization of Fluorescence,” Biomechanical Journal (1952) 51:165-77.
Levine, L.M. et al.,Anal. Biochem. (1997) 247:83-88.
Levison, S.A. et al., “Fluorescence Polarization Measurement of the Hormone-Binding Site Interaction,” Endocrinology (1976) 99:1129.
Lundblad, J.R., et al., “Fluorescence Polarization Analysis of Protein-DNA and Protein-Protein Interactions,” Molecular Endocrinology (1996) 10:607-612.
Lynch, B.A. et al., “A Fluorescence Polarization Based Src-SH2 Binding Assay,” Analytical Biochemistry (1997) 247:77-82.
Murakami, A. et al.,Nucl. Acids Res. (1991) 19:4097-4102.
Owicki, J.C. et al., “Application of Fluorescence Polarization Assays in High-Throughput Screening,” Genetic Engineering News (1997).
Ozers, M.S. et al., “Equilibrium Binding of Estrogen Receptor with DNA Using Fluorescence Anistropy,” J. Biol. Chem. (1997) 272:30405-11.
Schade, S.Z. et al., “BODIPY-a-Casein, a pH-Independent Protein Substrate for Protease Assays Using Fluorescence Polarization,” Analytical Biochemistry (1996) 243:1-7.
Smallshaw, J.C. et al., “Determination of the Binding Constants for Three HPr-Specific Monoclonal Antibodies and Their Fab Fragments,” Mol. Biol. (1998) 280:765-74.
Tanha, J. et al., “Thermodynamic Analysis of Monoclonal Antibody Binding to Duplex DNA,” Nucleic Acids Research (1997) 25:1442-49.
Tyagi, S, et al., Nature Biotech, (1996), 14:303-308.
Tyagi, S. et al., Nature Biotech, (1998), 16:49-53.
Walker, G.T. et al., “Strand Displacement Amplification (SDA) and Transient-State Fluorescence Polarization Detection of Mycobacterium Tuberculosis DNA,” Clinical Chemistry (1996) 42:9-13.
Walker, G.T. et al., Nucl. Acids Res. (1996), 24(2):348-353.
Weber, G. “Rotational Brownian Motion and Polarization of the Fluorescence of Solutions,” Adv. Protein Chem. (1953) 8:415.
Wei, A. et al., “Use of Synthetic Peptides as Tracer Antigens in Fluorescence Polarization Immunoassays of High Molecular Weight Analytes,” Anal. Chem. (1993) 65:3372.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Assay methods and systems does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay methods and systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay methods and systems will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3681080

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.