Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-01-27
1995-07-25
Fleisher, Mindy B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351723, 4352401, 436 63, 436 94, 935 36, 935 39, 935 41, C12Q 168, C12N 1563, G01N 3348, G01N 3300
Patent
active
054361281
ABSTRACT:
Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene under the transcriptional control of a promoter that is regulated, directly or indirectly, by the cell surface protein. The assays, provide a means for identifying potential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or antagonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are also provided.
REFERENCES:
patent: 4859609 (1989-08-01), Dull et al.
patent: 4981784 (1991-01-01), Evans et al.
patent: 5071773 (1991-12-01), Evans et al.
patent: 5091518 (1992-02-01), Sucov et al.
Collins et al., Proc. Natl. Acad. Sci., USA, 86 (Jul. 1989), pp. 4853-4857.
Herschman, TIBS 14, (Nov. 1989), 455-458.
Allard, et al., "Sequence of the gene encoding the human M1 muscarinic acetylcholine receptor," Nucl. Acids. Res., 15:10604 (1987).
Subramani, et al., "Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40," Mol. Cell Biol., 1:854-864 (1981).
Deschamps, et al., "Identification of a transcriptional enchancer element upstream from the proto-oncogen fos," Science, 230:1174-1177 (1985).
Michel, et al., "PC12 phaeochromocytoma cells contain an atypical muscarinic receptor binding site," Br. J. Pharmacol., 97:914-920 (1989).
Lambert, et al., "Muscarinic receptor binding characteristics of a human neuroblastoma SK-N-SH and its clones SH-SY5Y and SH-EP1," Eur. J. Pharmacol., 165:71-77 (1989).
Serra, et al., "The intact human neuroblastoma cell (SH-SY5Y) exhibits high-affinity [.sup.3 H]pirenzepine binding associated with hydrolysis of phosphatidylinositols," J. Neurochem., 50:1513-1521 (1986).
Serra, et al., "Phorbol esters alter muscarinic receptor binding and inhibit polyphosphoinositide breakdown in human neuoblastoma (SH-SY5Y) cells," Biochem. Biophys. Res. Comm., 140(1):160-166 (1986).
Peralta, et al., "Differential regulation of Pl hydrolysis and adenylyl cyclase by mascarinic receptor subtypes," Nature, 334:434-437 (1988).
Horwitz, et al., "Muscarinic receptor stimulation increases inositol-phospholipid metabolism and inhibits cyclic AMP accumulation in PC12 cells," J. Neurochem., 53:197-204 (1989).
Bonner, et al., "Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes," Neuron, 1:403-410 (1988).
Wada, et al., "Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor," Science, 240:330-334 (1988).
Boulter, et al., "Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor .alpha.-subunit," Nature, 319:368-374 (1986).
Goldman, et al., "Members of a nicotinic acetylcholine receptor gene family are expressed in different regions of the mammalian central nervous system", Cell, 48:965-973 (1987).
Boulter, et al., ".alpha.3, .alpha.5, and .beta.4: Three members of the rat neuronal nicotinic acetylcholine receptor-related gene family form a gene cluster," J. Biol. Chem., 265:4472-4482 (1990).
Deneris, et al., "Primary structure and expression of .beta.2: A novel subunit of neuronal nicotinic acetylcholine receptors," Neuron, 1:45-54 (1988).
Deneris, et al., ".beta..sub.3 : A new member of nicotinic acetylcholine receptor gene family is expressed in brain," J. Biol. Chem., 264:6268-6272 (1989).
Duvoisin, et al., "The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: .beta.4," Neuron, 3:487-496 (1989).
Schofield, et al., "Sequence and functional expression of the GABA.sub.A receptor shows a ligand-gated receptor super-family," Nature, 328:221-227 (1987).
Levitan, et al., "Structural and functional basis for GABA.sub.A receptor heterogeneity," Nature, 335:76-79 (1988).
Pritchett, et al., "Importance of a novel GABA.sub.A receptor subunit for benzodiazepine pharmacology," Nature, 338:582-585 (1989).
Ymer, et al., "GABA.sub.A receptor .beta. subunit heterogeneity: functional expression of cloned cDNAs," EMBO J., 8:1665-1670 (1989).
Shivers, et al., "Two novel GABA.sub.A receptor subunits exist in distinct neuronal subpopulations," Neuron, 3:327-337 (1989).
Hollmann, et al., "Cloning by functional expression of a member of the glutamate receptor family," Nature, 342:643-648 (1989).
Frielle, et al., "Cloning of the cDNA for the human .beta..sub.1 -adrenergic receptor," PNAS, 84:7920-7924 (1987).
Kobilka, et al., "Cloning, sequencing, and expression of the gene coding for the human platelet .alpha..sub.2 -adrenergic receptor," Science, 238:650-656 (1987).
Dixon, et al., "Cloning of the gene and cDNA for mammalian .beta.-adrenergic receptor and homology with rhodopsin," Nature, 321:75-79 (1986).
Stormann, et al., "Molecular cloning and expression of a dopamine D2 receptor from human retina," Molec. Pharm., 37:1-6 (1990).
Bunzow, et al., "Cloning and expression of a rat D.sub.2 dopamine receptor cDNA," Nature, 336:783-787 (1988).
Johnson, et al., "Expression and structure of the human NGF receptor," Cell, 47:545-554 (1986).
Kobilka, et al., "An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins," Nature, 329:75-79 (1987).
Julius, et al., "The 5HT2 receptor defines a family of structurally distnict but functionally conserved serotonin receptors," PNAS, 87:928-932 (1990).
Julius, et al., "Molecular characterization of a functional cDNA encoding the serotonin 1c receptor," Science, 241:558-564 (1988).
Tanabe, et al., "Primary structure of the receptor for calcium channel blockers from skeletal muscle," Nature, 328:313-318 (1987).
Ellis, et al., "Sequence and expression of mRNAs encoding the .alpha..sub.1 and .alpha..sub.2 subunits of a DHP-sensitive calcium channel," Science, 241:1661-1664 (1988).
Ruth, et al., "Primary structure of the .beta. subunit of the DHP-sensitive calcium channel from skeletal muscle," Science, 245:1115-1118 (1989).
Jay, et al., "Primary structure of the .gamma. subunit of the DHP-sensitive calcium channel from skeletal muscle," Science, 248:490-492 (1990).
McKinnon, D., "Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family," J. Biol. Chem., 264:8230-8236 (1989).
Tempel, et al., "Cloning of a probable potassium channel gene from mouse brain," Nature, 332:837-839 (1988).
Noda, et al., "Existence of distinct sodium channel messenger RNAs in rat brain," Nature, 320:188-192 (1986).
Kayano, et al., "Primary structure of rat brain sodium channel III deduced from the cDNA sequence," FEBS Lett., 228:187-194 (1988).
Fink, et al., "The CGTCA sequence motif is essential for biological activity of the vasoactive intestinal peptide gene cAMP-regulated enhancer," Proc. Natl. Acad. Sci., 85:6662-6666 (1988).
Montminy, et al., "Identification of a cyclic-AMP-responsive element within the rat somatostatin gene," Proc. Natl. Acad. Sci., 83:6682-6686 (1986).
Comb, et al., "A cyclic AMP- and phorbol ester-inducible DNA element," Nature, 323:353-356 (1986).
Short, et al., "Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region," J. Biol. Chem., 261:9721-9726 (1986).
Changelian, et al., "Structure of the NGFI-A gene and detection of upstream sequences responsible for its transcriptional induction by nerve growth factor," Proc. Natl. Acad. Sci., 86:377-381 (1989).
Visvader, et al., "Two adjacent promotor elements mediate nerve growth factor activation of the c-fos gene and bind distinct nuclear complexes," PNAS, 85:9474-9478 (1988).
Verma, et al., "Proto-Oncogene fos: Complex but versatile regulation," Cell, 51:513-514 (1987).
Sheng, et al., "The regulation and function of
Brust Paul
Harpold Michael M.
Fleisher Mindy B.
Salk Institute Biotechnology/Industrial Associates
LandOfFree
Assay methods and compositions for detecting and evaluating the does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assay methods and compositions for detecting and evaluating the , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay methods and compositions for detecting and evaluating the will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-738995