Electrolysis: processes – compositions used therein – and methods – Electrolytic analysis or testing – Involving enzyme or micro-organism
Patent
1994-02-22
1996-04-16
Beisner, William H.
Electrolysis: processes, compositions used therein, and methods
Electrolytic analysis or testing
Involving enzyme or micro-organism
435 25, 435817, 205782, C12Q 126, C12Q 154
Patent
active
055081711
ABSTRACT:
A new electrochemical biosensor and method of its use. The electrochemical biosensor has a pair of electrodes consisting of a working electrode and a counter electrode made of the same electrically conducting materials. The counter electrode may be the same size or smaller than the working electrode. The biosensor includes a sample receiving portion wherein a portion of the electrode surfaces is covered by a reagent that includes a redox mediator and an enzyme. A fluid that contains an analyte is added to the sample receiving portion. A reaction involving the analyte, enzyme and redox mediator occurs. After this reaction is complete, an electrical potential difference is applied between the electrodes. Diffusion limited current is measured and correlated to the concentration of analyte in the fluid.
REFERENCES:
patent: 4224125 (1980-09-01), Nakamura et al.
patent: 4543326 (1985-09-01), Miyashita et al.
patent: 4758323 (1988-07-01), Davis et al.
patent: 4810633 (1989-03-01), Bauer et al.
patent: 4820399 (1989-04-01), Senda et al.
patent: 4830959 (1989-05-01), McNeil et al.
patent: 4894137 (1990-01-01), Takizawa et al.
patent: 4897173 (1990-01-01), Nankai et al.
patent: 4959305 (1990-09-01), Woodrum
Talbott, Jonathan Lee, "Enzymatic Amperometry of Glucose", a Ph.D. Dissertation for The Pennsylvania State University, Graduate School Department of Chemistry, dated Jan. 1988.
Nankai, et al., Japanese Sensor Newsletter, vol. 3, No. 1, Mar./1989--p.p. 16-20, published by Edison Sensor Technology Center, Case Western Reserve University, Cleveland, Ohio.
Williams et al, "Electrochemical-Enzymatic Analysis of Blood Glucose and Lactate", 1970, vol. 42, No. pp. 118-121, Analytical Chemistry.
Bateson Joseph E.
Gerber Martin T.
Han Chi-Neng A.
Kost Kent M.
Kuhn Lance S.
Amick Marilyn L.
Beisner William H.
Boehringer Mannheim Corporation
Kenemore Max J.
Young D. Michael
LandOfFree
Assay method with enzyme electrode system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assay method with enzyme electrode system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay method with enzyme electrode system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-324424