Assay method with enzyme electrode system

Electrolysis: processes – compositions used therein – and methods – Electrolytic analysis or testing – Involving enzyme or micro-organism

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 25, 435817, 205782, C12Q 126, C12Q 154

Patent

active

055081711

ABSTRACT:
A new electrochemical biosensor and method of its use. The electrochemical biosensor has a pair of electrodes consisting of a working electrode and a counter electrode made of the same electrically conducting materials. The counter electrode may be the same size or smaller than the working electrode. The biosensor includes a sample receiving portion wherein a portion of the electrode surfaces is covered by a reagent that includes a redox mediator and an enzyme. A fluid that contains an analyte is added to the sample receiving portion. A reaction involving the analyte, enzyme and redox mediator occurs. After this reaction is complete, an electrical potential difference is applied between the electrodes. Diffusion limited current is measured and correlated to the concentration of analyte in the fluid.

REFERENCES:
patent: 4224125 (1980-09-01), Nakamura et al.
patent: 4543326 (1985-09-01), Miyashita et al.
patent: 4758323 (1988-07-01), Davis et al.
patent: 4810633 (1989-03-01), Bauer et al.
patent: 4820399 (1989-04-01), Senda et al.
patent: 4830959 (1989-05-01), McNeil et al.
patent: 4894137 (1990-01-01), Takizawa et al.
patent: 4897173 (1990-01-01), Nankai et al.
patent: 4959305 (1990-09-01), Woodrum
Talbott, Jonathan Lee, "Enzymatic Amperometry of Glucose", a Ph.D. Dissertation for The Pennsylvania State University, Graduate School Department of Chemistry, dated Jan. 1988.
Nankai, et al., Japanese Sensor Newsletter, vol. 3, No. 1, Mar./1989--p.p. 16-20, published by Edison Sensor Technology Center, Case Western Reserve University, Cleveland, Ohio.
Williams et al, "Electrochemical-Enzymatic Analysis of Blood Glucose and Lactate", 1970, vol. 42, No. pp. 118-121, Analytical Chemistry.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Assay method with enzyme electrode system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay method with enzyme electrode system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay method with enzyme electrode system will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-324424

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.