Assay method using nad synthetase and a process for production o

Details Assay method using nad synthetase and a process for production o Assay method using nad synthetase and a process for production o

1984-04-25

1988-08-30

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435836, C12N 120, C12R 110

Patent

active

047677120

ABSTRACT:
An assay method for a component in a specimen containing any one of ATP, deamide-NAD and an amide donor which comprises performing a main reaction which comprises incubating the specimen with NAD synthetase in the presence of ATP, deamide-NAD, an amide donor and Mg.sup.++ to generate NAD; performing a coenzyme-cycling reeaction by combining the oxidation-reduction reaction system with coenzyme NAD and the oxidation-reduction reaction system with coenzyme reduced NAD, and measuring a consumed or generated component in the cycling reaction. The NAD synthetase can be produced by culturing the microorganism Bacillus licheniformis B-0844 FERM P-6809, in a culture medium, and isolating the thus-produced NAD synthetase therefrom.

REFERENCES:
patent: 4318980 (1982-03-01), Boguslaski et al.
patent: 4427771 (1984-01-01), Misaki et al.
Bergmeyer, Methods of Enzymatic Analysis, vol. 1, 4, 2nd Ed. Academic Press, Inc., New York, 131-144, 1909, 1919 (1974).
Yu et al., Chemical Abstracts, 77: 110798p, 154 (1972).
Preiss et al., J. Biol. Chem., 233(2): 493-500 (1958).
Imsande et al., J. Biol. Chem., 236(2): 525-530 (1961).
Yu et al., J. Biol. Chem., 237: 4794-4802 (1972).
Imsande et al., J. Biol. Chem., 236: 1494-1497 (1961).
Spencer et al., J. Biol. Chem., 242(3): 385-392 (1967).

Affiliated with

Also associated with

Rating

Assay method using nad synthetase and a process for production o does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay method using nad synthetase and a process for production o, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay method using nad synthetase and a process for production o will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2088060