Assay method using NAD synthetase

Details Assay method using NAD synthetase Assay method using NAD synthetase

1990-02-15

1993-04-27

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 4, 435 15, 43525231, 435836, C12Q 100, C12Q 148, C12Q 120, C12N 100

Patent

active

052061469

ABSTRACT:
An assay method for a component in a specimen containing any one of ATP, deamide-NAD and an amide donor which comprises performing a main reaction which comprises incubating the specimen with NAD synthetase in the presence of ATP, deamide-NAD, an amide donor and Mg.sup.++ to generate NAD; performing a coenzyme cycling reaction by combining the oxidation-reduction reaction system with coenzyme NAD and the oxidation-reduction reaction system with coenzyme reduced NAD, and measuring a consumed or generated component in the cycling reaction. The NAD synthetase can be produced by culturing the microorganism Bacillus licheniformis B-0844 FERM P-6809, in a culture medium, and isolating the thus-produced NAD synthetase therefrom.

REFERENCES:
patent: 4318980 (1982-03-01), Boguslaski et al.
patent: 4427771 (1984-01-01), Misaki et al.
Bergmeyer, Methods of Enzymatic Analysis, vol. 1, 4, 2nd Ed., Academic Press, Inc. New York, 131-144, 1909, 1919 (1974).
Yu et al, Chemical Abstracts, 77:110798.
Preiss et al, J. Biol. Chem., 233(2): 493-500 (1958).
Imsande et al, J. Biol. Chem., 236(2): 525-530 (1961).
Yu et al, J. Biol. Chem., 247: 4794-4802 (1972).
Imsande et al, J. Biol. Chem., 236: 1494-1497 (1961).
Spencer et al, J. Biol. Chem., 242(3): 385-392 (1967).

Affiliated with

Also associated with

Rating

Assay method using NAD synthetase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay method using NAD synthetase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay method using NAD synthetase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2326572