Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1995-02-27
1998-12-01
Chan, Christina Y.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435219, 436811, C12Q 137, C12N 950
Patent
active
058436936
ABSTRACT:
Methods and materials are disclosed for the production of purified, active recombinant human neutrophil protease, PR-3, via activation of a pro-form herein referred to as proPR-3. Human PR-3 is useful for discovering inhibitors of excessive release of mature, active TNF.alpha.. Also disclosed are methods for the identification of inhibitors of the conversion of the pro-form of TNF.alpha. to its mature active form.
REFERENCES:
patent: 4179337 (1979-12-01), Davis et al.
patent: 4495285 (1985-01-01), Shimizu et al.
patent: 4582788 (1986-04-01), Ehrlich
patent: 4596822 (1986-06-01), Powers et al.
patent: 4609546 (1986-09-01), Hiratani
patent: 4677063 (1987-06-01), Mark et al.
patent: 4677064 (1987-06-01), Mark et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4699904 (1987-10-01), Doherty et al.
patent: 4704692 (1987-11-01), Ladner
patent: 4711886 (1987-12-01), Finke et al.
patent: 4717722 (1988-01-01), Doherty et al.
patent: 4766106 (1988-08-01), Katre et al.
patent: 4797396 (1989-01-01), Finke et al.
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 4829054 (1989-05-01), Emerson et al.
patent: 4923807 (1990-05-01), Webb et al.
patent: 4968614 (1990-11-01), Takiguchi et al.
patent: 5091303 (1992-02-01), Arnaout et al.
patent: 5109018 (1992-04-01), Powers et al.
patent: 5124147 (1992-06-01), Wissner et al.
patent: 5128258 (1992-07-01), Klostergaard et al.
patent: 5136021 (1992-08-01), Dembinski et al.
patent: 5180819 (1993-01-01), Cayre et al.
patent: 5200333 (1993-04-01), Wilson
patent: 5247070 (1993-09-01), Yamamada et al.
Abstract, Dialog Accession No. 06706182, Medline Accession No. 89008182, Oshawa F. et al.: "Selective degradation of tumor necrosis factor in sensitive cells, and production of membrane-active substance"; and J. Biochem. (Tokyo) (Japan) (Apr. 1988) 103(4): 730-4.
Natanson et al. (1994) Annals of Internal Medicine. vol. 120(9): 771-783.
Fox et al. (1989) Clin. Chem. vol. 35(9):1838-1842.
Abrams et al., "Optimal Strategies for Developing Human-Human Monoclonal Antibodies," Meth. Enzymol., 121:107-119 (1986).
Birnboim et al., "A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA," Nucleic Acids Research, 7:1513-1523 (1979).
Bories et al., "A Fertile Pair," Cell, 59:955-958 (Dec. 22, 1989).
Boss, B.D., "An Improved in Vitro Immunization Procedure for the Production of Monoclonal Antibodies," Meth. Enzym. 121:27-33 (1986).
Buck et al., "Monoclonal Antibodies Specific for Cell Culture Mycoplasmas," In Vitro, 18(4):377-381 (Apr., 1982).
Campanelli et al., "Cloning of cDNA for Proteinase 3: A Serine Protease, Antibiotic and Autoantigen from Human Neutrophils," J. Exp. Med., 172:1709-1715 (Dec., 1990).
Cohen et al., "Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA," Proc. Nat'l. Acad. Sci., USA, 69(8):2110-2114 (Aug., 1972).
Crawford et al., "Production of Human Monoclonal Antibody to X31 Influenza Virus Nucleoprotein," J. of General Virology, 64:697-700 (1983).
Croce et al., "Production of Human Hybridomas Secreting Antibodies to Measles Virus," Nature, 288:488-489 (1980).
Decker et al., "Cell-Associated Tumor Necrosis Factor (TNF) as a Killing Mechanism of Activated Cytotoxic Macrophages," J. of Immunol.,138(3):957-962 (Feb. 1, 1987).
Doherty et al., "Cephalosporin Antibodies Can Be Modified to Inhibit Human Leukocyte Elastase," Nature, 322:192-194 (Jul. 10, 1986).
Duff et al., "Tumor Necrosis Factor (TNF) and Interleukin 1 (IL 1) in Arthritis," International Conference on Tumor Necrosis Factor and Related Cytotoxins, 175:10 (1987).
Fendly, et al., "Murine Monoclonal Antibodies Defining Neutralizing Epitopes on Tumor Necrosis Factor," Hybridoma, 6(4):359-370 (1987).
Folks et al., "Tumor Necrosis Factor .alpha. Induces Expression of Human Immunodeficiency Virus in a Chronically Infected T-Cell Clone," Proc. Nat'l Acad. Sci. USA, 86:2365-2368 (Apr., 1989).
Graham et al., "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA," Virology, 52:456-467 (1973).
Ish-Horowicz et al., "Rapid and Efficient Cosmid Cloning," Nucleic Acids Res., 9(13):2989-2998 (1981).
Kam et al., "Substrate and Inhibitor Studies on Proteinase 3," FEBS, 297(1,2):119-123 (Feb., 1992).
Knauf et al., "Relationship of Effective Molecular Size to Systemic Clearance in Rats of Recombinant Interleukin-2 Chemically Modified with Water-soluble Polymers," J. Biol. Chem., 263(29):15064-15070 (Oct. 15, 1988).
Kohler et al., "Continuous Cultures of Fused Cells Secreting Antibody of Predefined Specificity," Nature, 256:495-497 (Aug. 7, 1975).
Kozbor et al., "The Production of Monoclonal Antibodies From Human Lymphocytes," J. Immun. Today, 4(3):72-79 (1983).
Kramer et al., "Oligonucleotide-Directed Construction of Mutations via Gapped Duplex DNA," Meth. Enzymol.,154:350-367 (1986).
Kriegler et al., "A Novel Form of TNF/Cachectin Is a Cell Surface Cytotoxic Transmembrane Protein: Ramifications for the Complex Physiology of TNF," Cell, 53:45-53 (Apr., 1988).
Kriegler et al., "Transformation Mediation by the SV40 T Antigens: Separation of the Overlapping SV40 Early Genes with a Retroviral Vector," Cell, 38:483-491 (Sep., 1984).
Larrick, J.W., "Plate Fusion Technique for Nonadherent Cells," Human Hybridomas and Monoclonal Antibodies, Engleman et al. (Eds.), Plenum Press, New York, pp. 446-448 (1985).
Luben et al., "In Vitro Immunization As An Adjunct to the Production of Hybridomas Producing Antibodies Against the Lymphokine Osteoclast Activating Factor," Molecular Immunology, 17:635-639 (1980).
Maki, D.G., "Nosocomial Bacteremia:An Expidemiologic Overview," Nosocomical Infect., Dikson, R.E., (Ed.), Yrke Medical Books, USA, pp. 183-196 (1981).
Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor Press, pp. 254-255 (1982).
Matteucci et al., "Synthesis of Deoxyoligonucleotides on a Polymer Support," J. Am. Chem. Soc., 103:3185-3191 (1981).
Maxam et al., "Sequencing End-Labeled DNA With Base-Specific Chemical Cleavages," Meth. Enzymol., 65:499-560 (1980).
Merrifield, B., "Solid Phase Synthesis," Science, 232:341-347 (1986).
Messing et al., "A System for Shotgun DNA Sequencing," Nucleic Acids Res., 9:309-321 (1981).
Navia et al., "Structure of Human Neutrophil Elastase in Complex With a Peptide Chloromethyl Ketone Inhibitor at 1.84-A Resolution," Proc. Nat'l Acad. Sci., USA, 86:7-11 (1989).
Oleksyszyn et al., "Irreversible Inhibition of Serine Proteases by Peptide Derivatives of (.alpha.-Aminoalkyl)Phosphonate Diphenyl Esters," Biochem., 30:485-493 (1991).
Rao et al., "Characterization of Proteinase-3 (PR-3), A Neutrophil Serine Proteinase," J. Biol. Chem., 266:9540-9548 (1991).
Reading, C.L., "Theory and Methods for Immunization in Culture and Monoclonal Antibody Production," J. of Immun. Methods, 53:261-291 (1982).
Reading, C.L., "In Vitro Immunization for the Production of Antigen-Specific Lymphocyte Hybridomas," Meth. Enzym., 121:18-27 (1986).
Roder et al., "The EBV-Hybridoma Technique," Meth. Enzym., 121:140-167 (1986).
Sanger et al., "DNA Sequencing With Chain-Terminating Inhibitors," Proc. Nat'l Acad. Sci., USA, 74:5463-5467 (1977).
Schlom et al., "Generation of Human Monoclonal Antibodies Reactive With Human Mammary Carcinoma Cells," Proc. Nat'l Acad. Sci., USA, 77:6841-6845 (1980).
Scuderi et al., "Cathepsin-G and Leukocytes Elastase Inactive Human Tumor Necrosis Factor and Lymphotoxin," Cellular Immunol., 135:299-313 (1991).
Seckinger et al., "Purification and Biologic Characterization of a Specific Tumor Necrosis Factor .alpha. Inhibitor," J. Biol. Chem., 264:11966-11973 (1989).
Seckinger et al., "A Human Inhibitor of Tumor Necrosis Factor .alpha.," J. Exp. Med., 167:1511-1516 (1988).
Smith, M., "In Vitro Mutagenesis," Annual Review of Genetics, 19:423-462 (1985).
Springer, T.A., "Cell-Surface Differentiation in the Mouse," Monoclonal Antibodies, Kennett et al., (Eds.), Plenum Press, New York, pp. 185-217 (1980).
Stetler et al., "Isolation and Sequence of a Human Gene Encoding a Potent Inhibitor of Leukocyte
Halenbeck Robert F.
Jewell David A.
Koths Kirston E.
Kriegler Michael
Perez Carl
Blackburn Robert P.
Cech Emma
Chan Christina Y.
Chiron Corporation
Pochopien Donald J.
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