Assay method for organic calcium antagonist drugs and a kit for

Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent

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436804, 436808, 436815, G01N 3360, G01T 100

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active

045201129

ABSTRACT:
A method for measuring the level of organic calcium antagonist drug in a body fluid comprises preparing a mixture of a radioactive calcium antagonist drug, a body fluid containing a calcium antagonist drug and a calcium antagonist receptor material, measuring the radioactivity of the radioactive calcium antagonist drug bound to said calcium antagonist receptor material and deriving the concentration of the calcium antagonist drug in the body fluid from a standard curve indicating the concentration of calcium antagonist drug versus inhibition of binding of said radioactive calcium antagonist drug to said receptor sites caused by the calcium antagonist drug in said body fluid. A kit for measuring the level of an organic calcium drug comprises a receptacle containing a radioactive calcium antagonist drug, a calcium antagonist receptor material and a standard amount of a nonradioactive calcium antagonist drug.

REFERENCES:
Murphy et al., Evr. J. Pharmacol 77 (1982) 201-202.
Murphy et al., Proc. Natl. Acad. Sci. USA 80 (1983) 860-864.
Gould et al., Proc. Natl. Acad. Sci. USA 79 (1982) 3656-3660.
McAllister et al., J. Pharmaceutical Sci., 65 (1976) 431-432.

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