Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol
Reexamination Certificate
2008-07-22
2008-07-22
Saucier, Sandra (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving cholesterol
C436S071000
Reexamination Certificate
active
11116469
ABSTRACT:
The present invention relates to an assay for determining net cholesterol flux from lipid loaded cells in the presence of an acceptor (e.g., serum). The present invention also relates to a method for evaluating efficacy of a therapeutic agent for stimulating or inhibiting net cholesterol flux. Also provided by the present invention are kits that are used to carry out the aforementioned assays and methods.
REFERENCES:
Stedman's Medical Dictionary, from Dictionary.com, accessed Oct. 18, 2007.
Rothblat et al., “Cell cholesterol efflux: integration of old and new observations provides new Insights”, J. Lipid Research 40 : 781-796 (1999).
Fofana, M., et al., “Transfer of cholesterol between high density lipoproteins and cultured rat Sertoli cells”, Biochem. Cell Biol., 74: 681-686 (1996).
Fournier, N., et al., “Fractional efflux and net change in cellular cholesterol content mediated by sera from mice expressing both human apolipoprotein Al and human lecithin: cholesterol acyltransferase genes”, Atherosclerosis, 147: 227-235 (1999).
Atger, V.M., et al., “Cyclodextrins as Catalysts for the Removal of Cholesterol from Macrophage Foam Cells”, J. Clin. Invest., vol. 99: 773-780, (1997).
Shio, H., et. al., “Characterization of Lipid-Laden Aortic Cells from Cholesterol-Fed Rabbits”; Lab. Invest. 39: 390-397 (1978).
Small, D. M., “Progression and Regression of Atherosclerotic Lesions”; Arteriosclerosis, 8: 103-129 (1988).
Contreras, J. A., et. al., “Essential Differences in Cholesteryl Ester Metabolism Between Human Monocyte-Derived and J774 Macrophages”; Arterioscler. Thromb. 14: 443-452 (1994).
Hakamata, H., et. al., “Species Difference in Cholesteryl Ester Cycle and HDL-Induced Cholesterol Efflux From Macrophage Foam Cells”; Arterioscler. Thromb. 14: 1860-1865 (1994).
Johnson, W. J., et. al., “Cholesterol transport between cells and high-density lipoproteins”; Biochim. Biophys. Acta. 1085: 273-298 (1991).
Hakamata, H., et. al., “Differential effects of an acyl-coenzyme A:cholesterol acyltransferase inhibitor on HDL-induced cholesterol efflux from rat macrophage foam cells”; FEBS 363: 29-32 (1995).
Johnson, W. J., et. al., “The Bidirectional Flux of Cholesterol between Cells and Lipoproteins”; J. Biol. Chem. 261: 5766-5776 (1986).
Bernard, D. W., et al., “cAMP Stimulates Cholesteryl Ester Clearance to High Density Lipoproteins in J774 Macrophages”; J. Biol. Chem. 266: 710-716 (1991).
Miyazaki, A., et. al., “Acetylated Low Density Lipoprotein Reduces Its Ligand Activity for the Scavenger Receptor after Interaction with Reconstituted High Density Lipoprotein”; J. Biol. Chem. 269: 5264-5269 (1994).
Kellner-Weibel Ginny
Rothblat George H.
Dann Dorfman Herrell & Skillman
Netter Robert C.
Rigaut Kathleen D.
Saucier Sandra
The Children's Hospital of Philadelphia
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