Assay method for lipid component, assay composition, and process

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 11, 435 15, 435 19, 435 20, 435 21, 435 25, 435 26, 435189, 435190, 435183, 435193, 435232, 435874, C12Q 100, C12Q 126, C12Q 132, C12Q 142, C12Q 144, C12Q 146, C12Q 148, C12Q 160, C12N 902, C12N 904, C12N 900, C12N 910, C12N 988, C12R 138

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044916310

ABSTRACT:
An enzyme having enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity and 3-ketoacyl-CoA thiolase activity, all in the same enzyme, is produced by culturing the microorganism strain Pseudomonas fragi B-0771 FERM-P No. 5701, and isolating the enzyme thus produced from the culture medium. Such an enzyme is useful in an assay method for a fatty acid component in a sample, which fatty acid is originally present in the sample or is liberated from a fatty acid ester in the sample, comprising:

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