Assay method for lecithin-cholesterol acyltransferase

Details Assay method for lecithin-cholesterol acyltransferase Assay method for lecithin-cholesterol acyltransferase

1990-07-09

1992-06-16

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 4, 435 11, 435 19, 435 20, 435 26, 435 28, 435810, C12Q 148, C12Q 100, C12Q 160, C12Q 144

Patent

active

051224542

ABSTRACT:
An assay with high sensitivity for activity of lecithin-cholesterol acyltransferase in blood for functional analysis of liver, by bringing the blood into contact with lecithin and free cholesterol until lysolecithin and cholesterol ester are produced, allowing the lysolecithin produced to react with lysophospholipase and glycerophosphocholine phosphodiesterase and assaying glycerol-3-phosphate produced simultaneously or successively in the reaction by means of an enzymatic cycling reaction in which glycerol-3-phosphate, dihydroxyacetone-3-phosphate, nicotinamide adenine dinucleotide (NAD), reduced NAD, O.sub.2, H.sub.2 O.sub.2, glycerophosphate oxidase and glycerophosphate dehydrogenase take part.

REFERENCES:
patent: 4370318 (1983-01-01), Umezawa et al.
patent: 4693971 (1987-09-01), Misaki
Biological Abstracts, vol. 64, No. 6, Nov. 15, 1977, p. 34415 Abstract No. 34407, Soler-Argilaga, C. et al. "Effect of Triton . . . ".
Lowenstein, J. M. Methods in Enzymology, vol. 71, part C pp. 753-767.
Shigeru Ueda, Hideo Misaki and Shigeyuki Imamura Japan Clinical Chemistry Abstract No. 232, vol. 32(6):1096 (1986).
Japan Clinical Chemistry, pp. 36 & 44 (1986).
Shigeru Ueda and Hideo Misaki, Japan Society of Clinical Chemistry 24:85 (1984).

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