Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1994-04-05
1996-03-05
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435 14, 435 22, 536 111, 536 186, 536102, 536122, 536127, 8473, 8916, 8921, 8930, 8931, 8934, C12Q 134, C07H 100
Patent
active
054967074
ABSTRACT:
The present invention provides an assay method for hemicellulases comprising a) directly dyeing, using a reactive dye, an insoluble natural product, or a modified form of a natural fibre material; and b) adding the enzyme to the dyed product produced in step a) and, after a specific incubation period, separating the liquid component from the insoluble dyed product, e.g. by a simple filtration, and determining the amount of dyestuff liberated in the the separated solution by spectrophotometric means. A combined mixing and dispensing device for use in the method of the present invention is also provided.
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Fisk Spencer
Freiermuth Beat
Gaertner Alfred
Werthemann Dieter
Ciba-Geigy Corporation
Genencor International Inc.
Mansfield Kevin T.
Mohamed Abdel A.
Wityshyn Michael G.
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