Assay method for detecting presence of bacteria

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 6, 435 731, 435 733, 435 735, 435 737, 435 792, 435 795, 435261, G01N 3302, G01N 33538, G01N 33547, G01N 33569

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056959460

ABSTRACT:
An assay method is provided to easily and quickly detect the presence of organisms capable of being cultured, such as bacteria, characterized by antibody capture of the organism of interest by use of specialized magnetic beads, incubation of the captured cells to form colonies; removal of material from the colonies with a colony lift membrane; and detection of the colony material on the membrane sheet by use of labeled antibodies, PCR or nucleic acid probes.

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