Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1994-11-29
1996-09-24
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435 18, 435 17, 435 21, 435 14, 435 12, 435 11, 435 10, 435 4, 436 63, 436 14, 436 13, 436 12, 436 74, 436 71, C12Q 128, C12Q 126, C12Q 154, G01N 3348
Patent
active
055590039
ABSTRACT:
The method is directed to assaying for biological components in a sample comprising step (A) generating an oxidase substrate in the presence of an amphoteric surfactant and in the absence of ferrocyanide; (B) initially generating hydrogen peroxide through said oxidase reaction on said substrate of oxidase with subsequent detection of the generated hydrogen peroxide using peroxidase and a color developer capable of being oxidized in the presence of amphoteric surfactant and ferrocyanide; and (C) correlating the amount of color developed to the amount of biological components in the biological sample. Even when the biological component to be detected is present in a very small amount, the interference of bilirubin can be eliminated in assays of biological components in which peroxidase generated from an enzymatic reaction is detected using peroxidase and a color developer capable of being oxidized.
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Abe Toshikatsu
Hosoi Kenji
Kawahara Sumi
Daiichi Pure Chemicals Co. Ltd.
Kight John
Leary Louise N.
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