Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is a biological cell or cell fragment
Patent
1984-08-28
1988-05-31
Marantz, Sidney
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is a biological cell or cell fragment
436520, 436536, 436537, 436800, 436805, 436807, 436829, 424 3, 424 11, G01N 33554, G01N 33555
Patent
active
047481293
ABSTRACT:
A method is provided for determining the presence in a sample of a member of a specific binding pair ("sbp member") consisting of ligand and its homologous receptor. The sample is combined in an aqueous medium with (1) a complementary sbp member wherein at least the sbp member or the complementary sbp member is bound to the surface of a cell and (2) a fuorescent agent capable of being incorporated into the cell. The presence of the sbp member is indicated by a change in fluorescence of the unseparated cell suspension as a result of agglutination of the cells.
The present invention has particular application to blood typing, for example, for the determination of the presence of blood group antigens A, B, AB, O, and D (Rh.sub.o) and antibodies to such antigens.
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Chang Chiu C.
Ghazarossian Vartan
Ullman Edwin F.
Leitereg Theodore J.
Marantz Sidney
Snytex (U.S.A.) Inc.
Wieder Stephen C.
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