Assay method and reagent kit means for lipid-containing body flu

Details Assay method and reagent kit means for lipid-containing body flu Assay method and reagent kit means for lipid-containing body flu

1981-02-17

1984-06-05

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436542, 436804, 436808, 436826, 436817, 422 61, G01N 3356, G01N 3358, G01N 3360, B65D 7100

Patent

active

044529031

ABSTRACT:
A method and reagent kit means are provided for assay of a selected hapten in an aliquot of body fluid containing lipid. The method comprises the steps of constituting the aliquot in a mixture with surfactant and incubating the mixture to solubilize the same. The content of hapten in the incubated mixture is taken up selectively by hapten-specific antibody and read by hapten non-lipid conjugate tracer assay.

REFERENCES:
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patent: 3997470 (1976-12-01), Monte et al.
patent: 4121975 (1978-10-01), Ullman et al.
patent: 4148869 (1979-04-01), Deaton
patent: 4208479 (1980-06-01), Zuk et al.
patent: 4235866 (1980-11-01), Thoma
patent: 4235869 (1980-11-01), Schwarzberg
The Merck Index, 8th Edition, Merck and Co., Rahway, N.J., 1968, pp. 848-849.
Engval et al., J. Immunol., 109(1), Jul. 1972, pp. 129-135.

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