Assay method and probe for polynucleotide sequences

Details Assay method and probe for polynucleotide sequences Assay method and probe for polynucleotide sequences

1983-02-01

1985-12-03

Hazel, Blondel

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436504, 436 63, 436 94, 436518, 436530, 435 6, 435 5, 435 7, 435 29, 435 34, 435 39, 935 78, G01N 3350, G01N 3354, C12Q 168, C12Q 170, C12Q 129, C12Q 104, C12Q 106

Patent

active

045566437

ABSTRACT:
An assay method for the detection of a specific nucleotide target sequence in a polynucleotide test extract is disclosed which utilizes a polynucleotide modified probe including both a cDNA sequence substantially complementary to the specific target sequence and a protein binding sequence. The assay is conducted by exposing the modified probe to the polynucleotide test extract for hybridization and then exposing the complex to the protein which binds to the protein binding sequence. An assay, such as an immunoassay, can then be conducted on the test sample to indicate the presence of the specific target sequence by detecting the presence of the binding protein.

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