Assay method and apparatus of immune reaction

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic

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422 68, 436805, 436806, 436807, 436518, G01N 33553

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active

049160818

ABSTRACT:
An immunoassay method and apparatus includes the vibration of beads within a cell, the beads having a complex of a substance conjugated with an enzyme as a marker on the surface thereof, and using an optical measurement device to quantatively determine the amount of the marker in the cell. The vibration is provided by including a magnetic material in the beads and causing a magnetic field to move within the cell. The vibrations have at least a component transverse to a measurement direction of the optical measurement device. The movement of the magnetic field in the cell is caused by the reciprocation of a bar having magnets thereon and in a position adjacent to the cell.

REFERENCES:
patent: 4092116 (1978-05-01), Giaever
patent: 4177253 (1979-12-01), Davies et al.
patent: 4452773 (1984-06-01), Molday
patent: 4454234 (1984-06-01), Czerlinski
patent: 4698302 (1987-10-01), Whitehead
patent: 4728500 (1988-03-01), Higo
patent: 4745077 (1988-05-01), Holian
Methods in Enzymology, vol. XXXIV, "Affinity Techniques", Covalent Linkage of Functional Ligands, and Proteins to Polyacrylamide Beads, John K. Inman, pp. 30-58, 1974.

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