Assay method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 5, 435 911, 436501, 436 94, C12Q 168, G01N 3348

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active

060226862

ABSTRACT:
Assay method for the detection of nucleic acid sequences in homogeneous solution. The method comprises the use of fluorescence polarisation to detect hybridisation of a fluorescent nucleic acid probe or to detect fluorescent primer extension products. Assay kits for use in the above methods.

REFERENCES:
patent: 4257774 (1981-03-01), Richardson et al.
patent: 4476228 (1984-10-01), Huchgermeier et al.
patent: 4668640 (1987-05-01), Wang et al.
patent: 4670380 (1987-06-01), Dattegupta
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4766062 (1988-08-01), Diamond et al.
patent: 4820630 (1989-04-01), Taub
patent: 4863876 (1989-09-01), Hevey
patent: 4959463 (1990-09-01), Froehler et al.
patent: 5185439 (1993-02-01), Arnold, Jr. et al.
Bloomfield et al. Physical Chemistry of Nucleic Acids Harper & Row NYNY (1974) pp. 456-460.
Morrison et al., "Solution-Phase Detection of Polynucleotides . . . ", Analytical Biochem., 183, 231-244(1989).
Urdea et al., "A comparison of non-radioisotopic hybridization . . . ", NAR, 16(11) 1988.
Asseline et al., "Oligodeoxynucleotide, covelently linked . . . ", EMBO, 3(4), 795-800, 1984.
Syranen et al., "Quantification of polymerase chain Reaction . . . ", NAR, 16(23) 1988.
Kobayashi, Tamiya and Karube (1988) a translation of the Preprint No. 1 IXC 18 entitled "New Assay Method for DNA Using Fluorescence Depolarization", contained in Collection 1 of Preprints of Lectures at the 56th Annual Spring Convention of Nihon Kagaku-Kai.
Matthews et al, "Review, Analytical Strategies for the Use of DNA Probes", Analytical Biochemistry, vol. 169, No. 1, Feb. 15, 1988, p. 1-25.
Gyllenstein et al, "Generation of Single-Stranded DNA by the Polymerase Chain Reaction and its Application to Direct Sequencing of the HLA-DQA Locus", Proc. Natl Acad. Sci. USA vol. 85, pp. 762-7656, Oct. 1988, Genetics.
Chemical Abstracts 105:204923z "Association of short DNA fragments: steady state fluorescence polarization study"; Hard et al & Bio-polymers 1986, vol. 25, No. 8, pp. 1519-1529.
Brumbaugh et al, "Continuous, on-line DNA Sequencing Using Oligodeoxynucleotide Primers with Multiple Fluorophores", Proc. Natl Acad Sci USA, vol. 85, pp. 5610-5614, Aug. 1988, Genetics.
Arnold, Jr. et al, "Assay Formats Involving Acridinium-Ester-Labeled DNA Probes," Clin. Chem. 3578, 1588-1594 (1989), vol. 35, No. 8, 1989.
Database WPIL/dERWENT, abstract No. 86-343471 (52), 1986, Derwent Publications Ltd., London, GB; & JP-A-61258168 (Ind. Res. Inst. of Japan) 15.ll.1986.
Morrisen et al, Solhon-Phase Delzehan/polynucleotides. Analytical Biochem. 183, 231-244 (1989).
Urdea, et al, A comparison of non- . . . isotopic/Bridgeham NAR, 16 (11) 1988.
Asseline et al otzoch . . . pro . . . EMBO, 34) 795-800, 1984.
Syvanen et al, Nucleic Acids Res., v. 16, 1988, 11327-38.
S. Kobayashi et al., New Assay Method for DNA Using Fluorescence Depolarization, Proc. Chemical Sensors, 1987, p. 370.
W.B. Dandliker et al., "Fluorescence Polarization Immunoassay, Theory and Experiment Method", Immunochemistry, 1973, vol. 10, pp. 219-227.
Brumbaugh et al, "Continuous, on-line DNA Sequencing Using Oligodeoxynucleotide Primers with Multiple Fluorophores", Proc. Natl Acad Sci USA, vol. 85, pp. 5610-5614, Aug. 1988, Genetics.
Arnold, Jr. et al, "Assay Formats Involving Acridinium-Ester-Labeled DNA Probes," Clin. Chem. 3578, 1588-1594 (1989), vol. 35, No. 8, 1989.
Database WPIL/dERWENT, abstract No. 86-343471 (52), 1986, Derwent Publications Ltd., London, GB; & JP--A--61258168 (Ind. Res. Inst. of Japan) 15.11.1986.

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