Chemistry: analytical and immunological testing – Involving production or treatment of antibody – Monoclonal antibody
Patent
1984-02-15
1988-02-23
Marantz, Sidney
Chemistry: analytical and immunological testing
Involving production or treatment of antibody
Monoclonal antibody
435 7, 43524026, 435805, 435810, 436513, 436530, 436808, 436809, 436810, 935103, 935110, G01N 3353, G01N 33543, G01N 33577
Patent
active
047270373
ABSTRACT:
A method of rapid determination of the isotype class for a panel of monoclonal antibodies is described. The assay comprises adsorbing on a solid support medium antibodies directed to specific immunoglobulin heavy and light chains. Once such isotype-specific antibodies are bound to the nitrocellulose paper, the treated strips can be incubated with the monoclonal antibody of interest. Upon formation of a complex between the specific iso-type antisera and the monoclonal antibody, the complex is visualized by reaction with a chromogenic substance. In the preferred embodiment of the invention, the treated nitrocellulose strips are stored in kit form. Using these prepared strips, the isotyping assay can be performed in less than two hours with a minimum of technical manipulation and expenditure of reagents.
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Cetus Corporation
Halluin Albert P.
Lauder Leona L.
Marantz Sidney
Nyari Linda J.
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