Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-02-28
1996-07-30
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 435962, 436518, 436 63, 436 537, 436540, 422 57, 422 58, 422100, 422101, G01N 33535, G01N 33536, G01N 33538, G01N 3374
Patent
active
055410693
ABSTRACT:
An assay for determining the presence of a threshold concentration of an analyte in a sample comprises first reacting the sample with an amount of anti-analyte selected to reduce the free analyte to a marginally detectable concentration. The sample is then contacted with anti-analyte immobilized on a test region or indicator zone on a solid phase, whereby the residual free analyte may be bound. The binding of free analyte to immobilized anti-analyte is detected by a variety of techniques to indicate the presence of the threshold concentration. By employing limited amounts of anti-analyte on the solid phase, the change between maximum binding of label and no binding of label will be responsive to very small changes in the analyte concentration originally present in the sample.
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patent: 5079170 (1992-01-01), Rosman et al.
D. Catty et al "ELISA and Related Enzyme Immunoassays" in Antibodies vol. II a Practical Approach Ed. D. Catty 1989 IRL Press pp. 97-154.
Mortensen Richard B.
Tom Henry K.
Housel James C.
Parsons Nancy
Quidel Corporation
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