Assay for the free portion of substances in biological fluids

Details Assay for the free portion of substances in biological fluids Assay for the free portion of substances in biological fluids

1980-09-22

1982-12-28

Nucker, Christine M.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436503, 436504, 424 1, G01N 3300

Patent

active

043661432

ABSTRACT:
A method of determining the concentration of the free portion of a ligand present in a biological fluid which also contains the ligand bound to one or more natural binders involves the single step of mixing a sample of the fluid with a labelled derivative of the ligand and a specific binder for the ligand, incubating the mixture, and determining the proportion of the labelled derivative of the ligand bound to the specific binder. The labelled derivative of the ligand is chosen to bind strongly to the added specific binder, but to bind not at all, or much more weakly than does the ligand, to the natural binders in the biological fluid. A small amount of the labelled derivative can be used, and the binding equilibrium of the ligand is not appreciably perturbed. Ligands include thyroxine, tri-iodothyroxine and cortisol.

REFERENCES:
patent: 4046870 (1977-09-01), Hertl et al.
patent: 4069305 (1978-01-01), Polito et al.
patent: 4128629 (1978-12-01), Eldred et al.
patent: 4225574 (1980-09-01), Romelli et al.
patent: 4235865 (1980-11-01), Thoma
Geokas et al., J. Biol. Chem., 1977, 252, 61-67.
Borgstrom et al., Scand. J. Clin. Labs. Invest., 1976, 36, 809-814.
Geokas et al., American Journal of Physiology, 1979, 236, Part 1, 77-83.

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