Assay for the diagnosis of flaviviral infection using...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S005000, C424S218100, C424S147100

Reexamination Certificate

active

11017048

ABSTRACT:
The invention concerns a method for early detection of a flavivirus-induced infection, comprising the detection of the flavivirus non-structural glycoprotein NS1 in a biological sample during the clinical phase of the infection, by an immumological method using at least two identical or different antibodies, the first antibody consisting of polyclonal or monoclonal antibodies pre-selected for their high affinity for said NS1 protein hexameric in shape.

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Crooks, Alan J. et al., , “The NS1 Protein of Tick-Borne Encephalitis Virus Forms Multimeric Species Upon Secretion From The Host Cell” Journal of General Virology (1994), 75. pp. 3453-3460.
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Falconar, A.K.I., et al., “Production of Dimer-Specific and Dengue Virus Group Cross-Reactive Mouse Monoclonal Antibodies to the Dengue 2 Virus Non-Structural Glycoprotein NS1”, Short Communication, (1991) pp. 962-965.
Falconar, A.K.I., “Immunoaffinity Purification of Native Dimer Forms of the Flavivirus Non-Structural Glycoprotein, NS1”, Journal of Virological Methods, 30 (1990) pp. 323-332.
Jacobs, S.C., et al., “High-Level Expression of the Tick-Borne Encephalitis Virus NS1 Protein by Using an Adenovirus-Based Vector: Protection Elicited in a Murine Model”, Journal of Virology, Apr. 1992 pp. 2086-2095.
Flamand, M. et al., “Purification and Renaturation of Japanese Encephalitis Virus Nonstructural Glycoprotein NS1 Overproduced by Insect Cells”, Protein Expression and Purification 6, (1995) pp. 519-527.
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