Assay for sperm quality

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 29, 435960, 436 63, 436906, C12Q 102, G01N 3353

Patent

active

061034819

ABSTRACT:
A method for evaluating sperm quality is disclosed. The first step of the method is to obtain a quantity of sample mammalian sperm and a sample of mature mammalian oocytes. The oocytes are fertilized with the sperm and the cumulus cells are removed from the fertilized oocytes. The oocytes are then labelled with antibodies directed to microtubules and the labelled microtubules are observed microscopically. The microtubule pattern is evaluated for microtubule aster size and organization. In one preferred from of the present invention, the mammalian sperm and mammalian oocytes are of the same species. In another preferred form of the present invention, the mammalian sperm and oocytes are of different species.

REFERENCES:
patent: 5200398 (1993-04-01), Strasberg et al.
patent: 5219729 (1993-06-01), Hodgen
Navara et al, Molec. Biol. of the Cell., 4, (Suppl.), p. 142 A, Abstr. No. 828, 1993.
CALBIOCHEM Immunochemicals, p. 424 (1993).
Chemical Abstracts, vol. 110, Draber, P., et al., "Differences in the Exposure of C- and N-terminal Tubulin Domains in Cytoplasmic Microtubules Detected with Domain-Specific Monoclonal Antibodies," Abstract 110: 190089w, Mammalian Biochem., 110: 495 (1989).
Holy, J., et al., "Spindle Pole Centrosomes of Sea Urchin Embryos Are Partially Composed of Material Recruited from Maternal Stores," Dev. Biol., 147:343-353 (1991).
ICN Biomedicals, Inc., p. IB-3 (1993).
Le Guen, et al., "Microtubule and Centrosome Distribution During Sheep Fertilization," Eur. J. Cell Biol., 48: 239-249 (1989).
Navara, C.S., et al., "Microtubule Organization in the Cow During Fertilization, Polyspermy, Parthenogenesis, and Nuclear Transfer: The Role of the Sperm Aster," Dev. Biol.,162:29-40 (1994).
Schatten, G., "The Centrosome and Its Mode of Inheritance: The Reduction of the Centrosome during Gametogenesis and Its Restoration during Fertilization," Dev. Biol., 165:299-335 (1994).
Schatten, G., et al., "Acetylated .alpha.-Tubulin in Microtubules during Mouse Fertilization and Early Development," Dev. Biol., 130: 74-86 (1988).
Schatten, G., et al., "Maternal Inheritance of Centrosomes in Mammals? Studies on Parthenogenesis and Polyspermy in Mice," Proc. Natl. Acad. Sci. USA, 88: 6785-6789 (1991).
Senn, A., et al., "Immunofluorescence Study of Actin, Acrosin, Dynein, Tubulin and Hyaluronidase and Their Impact on In Vitro Fertilization," Human Reproduct., 7: 841-849 (1992).
Sigma Immunochemicals, p. 89 (1993).
Simerly, C., et al., "The Paternal Inheritance of the Centrosome, the Cell's Microtubule-Organizing Center, in Humans, and the Implications for Infertility," Nature Medicine, 1: 47-52 (1995).
Simerly, C., et al., "Microinjected Kinetochore Antibodies Interfere with Chromosome Movement in Meiotic and Mitotic Mouse Oocytes," J. Cell Biol., 111:1491-1504 (1990).
Sirard, M.A., et al., "The Culture of Bovine Oocytes to Obtain Developmentally Competent Embryos," Biol. of Reproduct., 39: 546-552 (1988).
Stearns, T., "News and Views Article for Simerly et al. Paper," Nature Medicine, 1: 1-3 (1994).
Yanagimachi, R., et al., "The Use of Zona-Free Animal Ova as a Test System for the Assessment of the Fertilizing Capacity of Human Spermatozoa," Biol. of Reproduct.,15:471-476 (1976).
Zymed Laboratories, Inc., p. 49 (1993).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Assay for sperm quality does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay for sperm quality, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay for sperm quality will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2005177

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.