Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2011-04-19
2011-04-19
Kim, Young J (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S091100
Reexamination Certificate
active
07927841
ABSTRACT:
A family of PCR assays is disclosed for determining, both qualitatively and quantitatively, presence of material from a predetermined species source and for quantifying the amount of such material. The assays are based respectively on SINEs uniquely characteristic of pig species, cow species, chicken species, and ruminant sub-order, and having a high copy number. The assays disclosed permit rapid, inexpensive evaluation of meat samples to facilitate elimination from their diet of pork or beef by persons desiring to avoid such food sources; as well as the assay of cattle feed to determine presence therein of ruminant-source proteins, which are a potential source of bovine spongiform encephalopathy (BSE), commonly referred to as “mad cow disease.” The assays amplify the predetermined unique SINEs and the resulting amplified mixture is then evaluated qualitatively by electrophoresis on gel containing ethidium bromide or quantitatively by SYBR Green-based detection or TaqMan chemistry. The invention also extends to kits, primers, and other products used in connection with the assays. The amplicons are selected to be from about 100 to 170 bp long.
REFERENCES:
patent: 6174670 (2001-01-01), Wittwer et al.
patent: 2004/0146912 (2004-07-01), Lipton et al.
Walker et al. (Anal. Biochem. (May 2003) 316: 259-269).
Tajima et al. (Biosci. Biotechnol. Biochem. (Oct. 2002) 66(10): 2247-2250.
Verkaar et al. (Meat Science (2002) 60: 365-369).
Genbank Accession No. V00116 (1983).
Buck et al. (Biotechniques (1999) 27(3): 528-536).
Batzer Mark A.
Shewale Jaiprakash G.
Sinha Sudhir K.
Walker Jerilyn A.
Board of Supervisors of Louisiana State University and Agricultu
Bushnell , Esq. Robert E.
Kim Young J
Life Genetics Lab, LLC.
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