Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1991-09-20
1993-12-21
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435805, 435810, 436801, 436807, 436170, 436 63, 422 61, C12Q 146
Patent
active
052720616
ABSTRACT:
A dry analytical element for the determination of serum cholinesterase (CHE) activity is disclosed. The element analyses undiluted body fluids and employs butyrylthiocholine as the substrate for CHE. Butyrylthiocholine is hydrolyzed by serum cholinesterase and liberates butyric acid and thiocholine. The thiocholine liberated then reduces ferricyanide to ferrocyanide and the rate of change is measured by reflectance densitometry.
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Thomsen T., et al., Estimation of Cholinesterase Activity (EC 3.1.1.7;3.1.1.8) in Undiluted Plasma and Erythrocytes as a Tool for Measuring In Vivo Effects of Reversible Inhibitors, J. Clin. Chem. Clin. Biochem., vol. 26, pp. 469-475 (1988).
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Eastman Kodak Company
Everett John R.
Griffin Ronald W.
Webber Pamela S.
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