Chemistry: physical processes – Physical processes – Crystallization
Patent
1977-10-18
1980-12-23
Marantz, Sidney
Chemistry: physical processes
Physical processes
Crystallization
23905, 23911, 26032646, 548215, 548225, 548233, 546223, 546248, G01N 2348, G01N 2400
Patent
active
042407974
ABSTRACT:
Spin labels, especially dianionic aromatic spin labels which can bind to the first specific high-affinity bilirubin-binding site on serum albumin, and which are quantitatively displaceable into solution from said site in the presence of bilirubin, can be used in an assay for bilirubin-binding capacity. In the assay, an excess of spin label is mixed with the serum albumin or serum and is titrated with standard bilirubin. ESR spectroscopy indicates a change in the rate of spin label release with addition of bilirubin, giving a value indicative of bilirubin-binding capacity. Novel dianionic aromatic spin labels are also disclosed.
REFERENCES:
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patent: 3706537 (1972-12-01), Becher
patent: 3716335 (1973-02-01), Ullman
patent: 3730687 (1973-05-01), Rondeau
patent: 3915641 (1975-10-01), Goering
G. B. Odell, J. Pediat., 68, 164-180, (1966).
E. G. Porter, et al., J. Lab. Clin. Med., 67, 660-667, (1966).
C. L. Hamilton, et al., "Spin Labels", Chapt. in Structural Chemistry and Molecular Biology, A. Rich, et al., eds., pp. 115-147, W. H. Freeman Co., 1968.
J. C. Hsia, et al., J. Immunol. Meth. 3, 17-24, (1973).
Marantz Sidney
The Governing Council of the University of Toronto
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