Assay for qualitatively and/or quantitatively measuring hLH or h

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.

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424 88, 514 8, 530397, 530398, 530412, 530413, C07K 302, C07K 314, C07K 320, C07K 328

Patent

active

048809144

ABSTRACT:
A method to obtain a substantially pure and stable hLH-hCG receptor from gonadal cells is disclosed. The characteristics of the purified receptor are disclosed. The receptor, in addition to being used as receptor in an enzyme- or dye-receptor assay for hLH and/or hCG, can be used to purify hLH or hCG used in the preparation of standard dye-hormone or enzyme-hormone complexes. These complexes can be used in enzyme- or dye-immunoassays, as well as the receptor assay.

REFERENCES:
Metsikko, K., et al., FEBS Lett., 106(1), 193-196, (1979).
Dufau, M. L. et al., J. Biol. Chem., 250(12), 4822-4824, (1975).
Wimalasend, J. et al., J. Biol. Chem., 261(20), 9416-9420, (1986).

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