Assay for proteins and polypeptides

Details Assay for proteins and polypeptides Assay for proteins and polypeptides

1978-04-27

1980-08-26

Scovronek, Joseph

Chemistry: physical processes

Physical processes

Crystallization

252408, 422 56, G01N 3316, G01N 2102

Patent

active

042193370

ABSTRACT:
An assay for proteins or polypeptides in solution using a reagent of Coomassie Brilliant Blue G250 dye in perchloric or hydrochloric acid. Upon mixture of the acidic reagent with a protein sample, the dye couples with the protein and undergoes a color change, following which the absorbance of the mixture can be measured to provide a quantitative analysis for the protein in the sample; the same procedure may be used for sample solutions containing polypeptides. Proteins and polypeptides with molecular weights above about 2000-3000 may be assayed.

REFERENCES:
patent: 2785057 (1957-03-01), Schwab et al.
patent: 3980437 (1976-09-01), Kishimoto et al.
Reisner et al., The Use of Coomassie Brilliant Blue G250 Perchloric Acid Solution for Staining in Electrophoresis and Isoelectric Focusing on Polyacrylamide Gels, Anal. Biochem., vol. 64, pp. 509-516 (1975).

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