Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1995-01-17
1996-11-05
Paden, Carolyn
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 24, 435 34, 435805, 435810, 422 61, C12Q 134, C12N 1108, G01N 2100
Patent
active
055716849
ABSTRACT:
The presence of an enzymatically active hydrolase in a fluid sample is detected by contacting the sample with a solid-phase conjugate which is susceptible to cleavage by the hydrolase, and simultaneously or shortly thereafter, contacting the sample with an indicator which undergoes a detectable change upon the action of a reporter group. The reporter group is part of the conjugate and is liberated from it either partly or entirely by the action of the hydrolase. The indicator is susceptible to action by the reporter group only upon decoupling of the reporter group from the remainder of the conjugate, the decoupling occurring either in part or entirely upon action of the hydrolase. Also provided by this invention are various forms of a dry, self-contained test device which contains the conjugate described above plus the indicator and all other reagents and components necessary to achieve a detectable indication of the presence or absence of a catalytically active hydrolase. Preferred embodiments of the device also contain positive and negative controls.
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Andreasen Terrence J.
Lawrence Paul J.
Shockey David R.
Litmus Concepts Inc.
Paden Carolyn
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