Assay for polynucleotides employing oligonucleotides to eliminat

Details Assay for polynucleotides employing oligonucleotides to eliminat Assay for polynucleotides employing oligonucleotides to eliminat

1993-03-31

1995-07-18

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, 536 243, C12Q 168, C07H 2104

Patent

active

054340470

ABSTRACT:
In an assay for a target nucleotide sequence employing hybridization with a target probe nucleotide sequence, cross reaction between the target probe and a non-target nucleotide sequence, capable of hybridizing with the target probe, is prevented or diminished by introducing, at some point during the hybridization, a non-target probe which hybridizes preferentially with the non-target nucleotide sequence. The melting temperature for the non-target probe
on-target nucleotide sequence hybrid is preferably greater than that of the target probe
on-target sequence hybrid. The hybridization can be carried out under isothermal conditions or preferentially with gradual cooling.

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