Assay for nucleic acid sequences, particularly genetic lesions,

Details Assay for nucleic acid sequences, particularly genetic lesions, Assay for nucleic acid sequences, particularly genetic lesions,

1984-11-23

1989-04-11

Brown, Johnnie R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

935 78, 436501, 436 63, 436800, 435 6, 435803, 435 28, 435 26, 435 14, C12Q 168, C12Q 170, C12Q 128, C12Q 132

Patent

active

048206300

ABSTRACT:
Lesions in genetic sequences, for example, the sickle cell anemia mutation in the beta globin gene, are detected by means of interactive labels in a nucleic acid hybridization assay. A signal is generated only if the labels are in physical proximity which is made dependent on whether a predetermined normal or abnormal sequence is present by exposure to appropriate conditions such as restriction enzyme digestion or stringent hybridization.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4374925 (1983-02-01), Litman et al.
patent: 4376165 (1983-03-01), Hornby et al.
patent: 4378428 (1983-03-01), Farina et al.
patent: 4395486 (1983-07-01), Wilson et al.
patent: 4463090 (1984-07-01), Harris
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4656127 (1987-04-01), Mundy
patent: 4663278 (1987-05-01), DiNello
patent: 4683194 (1987-07-01), Saiki et al.
Renz, M. et al., Nucleic Acids Research, vol. 12, No. 8, Apr. 25, 1984, pp. 3435-3444.
Roninson, I. B. et al., Nature, vol. 309, Jun. 14, 1984, pp. 626-628.
Chemical Abstracts, vol. 98, No. 17, 1983, p. 280, Abstract No. 139894m, Patel, A. et al., "Chemiluminescence Energy . . . in Living Systems".
Cox, M. M. et al., Proc. Natl. Acad. Sci. USA, vol. 78, 1981, p. 3433.
Das Gupta, C. et al., Proc. Natl. Acad. Sci. USA, vol. 79, 1982, p. 762.
Bianchi, M. E. et al., Cell, vol. 35, 1983, p. 511.
Das Gupta, C. et al., Cell, vol. 22, 1980, p. 437.
Bianchi, M. et al., Cell, vol 34, 1983, p. 931.
B. Conner et al., Proc. Natl. Acad. Sci. USA, 80:278-282 (1983).
Orkin et al., Chem. Abstracts, 101:70382d (1984), p. 456.
Orkin et al., Concise Report, 64:311-313 (1984).

Affiliated with

Also associated with

Rating

Assay for nucleic acid sequences, particularly genetic lesions, does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay for nucleic acid sequences, particularly genetic lesions, , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay for nucleic acid sequences, particularly genetic lesions, will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-666806