Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1991-10-04
1994-09-20
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 243, 935 78, 435 29, 435 34, 435 35, 435 36, 436 63, 436501, C12Q 168
Patent
active
053488555
ABSTRACT:
A method for detecting (i) one or more microorganisms or (ii) nucleic acid sequences from a prokaryotic source or an eukaroytic source in an unpurified nucleic acid-containing test sample comprising
(a) labeling the nucleic acids in the test sample,
(b) contacting, under hybridization conditions, the labeled hybridizable nucleic acid and one or more immobilized hybridizable nucleic acid probes comprising (i) one or more known microorganisms or (ii) sequences from eukaroytic or prokaryotic sources, to form hybridized labeled nucleic acids, and
(d) assaying for the hybridized nucleic acids by detecting the label. The method can be used to detect genetic disorders, e.g., sickle-cell anemia.
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Dattagupta Nanibhushan
Huguenel Edward D.
Rabin Daniel U.
Rae Peter M. M.
Horlick Kenneth R.
Miles Inc.
Parr Margaret
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