Assay for Mycoplasma fermentans

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435199, 435810, 435870, C12R 135, C12Q 134, C12N 922

Patent

active

050734865

ABSTRACT:
An assay for detecting the presence of Mycoplasma fermentans is disclosed, along with a diagnostic kit that uses the assay. The presence of Mycoplasma fermentans is indicated by the presence of the restriction endonuclease MfeI. This endonuclease recognizes the sequence CAATTG and cleaves between the C and the first A.

REFERENCES:
Yanofsky, M. F., et al. (1986) J. Bacteriol. 168(1), 244-250.
Haden et al., Nucleic Acids Research, "Identification of a novel site specific endonuclease produced by Mycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines", vol. 17, No. 9, 1989, pp. 3491-3499.

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