Textiles: fiber preparation
Patent
1976-05-24
1978-01-03
Tanenholtz, Alvin E.
Textiles: fiber preparation
C12K 100
Patent
active
040665100
ABSTRACT:
An assay is disclosed for determining mutagenic damage caused by the administration of a known or suspected mutagen to diploid human lymphoblastoid cell lines. After administration of or exposure to a mutagenic agent, the lymphoblasts are incubated for a sufficient number of generations to allow full expression of phenotypic resistance to 6-thioguanine or other purines which serve as substrates for hypoxanthine guanine phosphoribosyl transferase (HGPRT). A surprising discovery is the remarkably long length of time required for such phenotypic expression. After the phenotypic lag has passed, the mutant fraction can be determined to complete the assay. A nontoxic, active, sterile microsomal drug-metabolizing system compatible with mammalian cell bioassays is also disclosed which can be used in the assay to determine metabolite-caused mutagenesis. These bioassay systems can be used by genetic toxicologists to determine the potential genetic hazards to human beings for a variety of suspected or known mutagens, including newly developed chemicals.
REFERENCES:
rothblat et al., Growth, Nutrition and Metabolism of Cells in Culture, pp. 238, 239 and 329, vol. 1, (1972).
Brook David E.
Massachusetts Institute of Technology
Shaw Robert
Smith, Jr. Arthur A.
Tanenholtz Alvin E.
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