Assay for motile facultative anaerobic pathogens

Details Assay for motile facultative anaerobic pathogens Assay for motile facultative anaerobic pathogens

1993-09-10

1995-04-11

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

435 25, 435244, 4352521, 4352536, 435801, C12N 100, C12Q 126

Patent

active

054057736

ABSTRACT:
An approved assay for the determination for motile facultative anaerobic pathogens is described which involves the use of a growth-enhancing substance such as oxyrase enzyme in order to increase the growth rate of a target pathogen in a growth medium, to thereby shorten the time required to complete the assay. The assay of the invention is particularly suited for the determination of L. monocytogenes in food products such as meat and dairy goods, and provides an effective screening assay which can be completed in about 24-36 hours. Preferably, a product sample is first incubated in Fraser Broth containing oxyrase enzyme; if a darkened color results, a small portion of the liquid is then placed within a U-shaped culture tube provided with modified Oxford agar; the motility characteristics of the target pathogen are observed in the U-tube, which is indicative of the presence of L. monocytogenes.

REFERENCES:
patent: 4996073 (1991-02-01), Copeland et al.
patent: 5187070 (1993-02-01), Fung et al.
Compiled by Jesse Ostnoff "FSIS Method for Isolation and Identification of Salmonella, Listeria Monocytogenes, and Campylobacter Jejuni in Meat and Poultry Carcasses" National Agricultural Library, Sep. 1989.
Cassiday et al., Applied and Environmental Microbiology, vol. 56, No. 7, pp. 2274-2275, Jul. 1990.
Oxyrase Inc., Technical bulletin, "Properties of the Oxyrase Enzyme System Used to Isolate and Cultivate Anaerobic Microorganisms" Ashland, Ohio 44805.
Adler, Critical Reviews in Biotechnology, vol. 10, No. 2, pp. 119-127, 1990.
Adler et al, J. Bacteriology, vol. 147, No. 2, pp. 326-332, Aug. 1981.

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